Immunodetection of specific Vibrio bacteria attaching to tissues of the giant tiger prawn Penaeus monodon

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Immunodetection of specific Vibrio bacteria attaching to tissues of the giant tiger prawn Penaeus monodon

An in vitro study of the attachment of Vibrio pathogens to tissues of the giant tiger prawn Penaeus monodon (Fabricius, 1798) was carried out using species-specific and genus-specific monoclonal antibodies with indirect FITCimmunofluorescence microscopy. The attachment of V. alginolyticus and V. parahaemolyticus to different tissues was always stronger than the attachment of V. anguillarum, and...

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Isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, Penaeus monodon.

Bacteria phenotypically resembling members of the phylogenetically distinct planctomycete group of the domain Bacteria were isolated from postlarvae of the giant tiger prawn, Penaeus monodon. A selective medium designed in the light of planctomycete antibiotic resistance characteristics was used for this isolation. Planctomycetes were isolated from both healthy and monodon baculovirus-infected ...

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Identification of Major and Minor Allergens of Black Tiger Prawn (Penaeus monodon) and King Prawn (Penaeus latisulcatus).

BACKGROUND Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of this study was to identify the IgE-binding proteins of these 2 prawn species. METHODS Raw and boiled prawn extracts were...

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Inhibition of luminescence and virulence in the black tiger prawn (Penaeus monodon) pathogen Vibrio harveyi by intercellular signal antagonists.

Expression of luminescence in the Penaeus monodon pathogen Vibrio harveyi is regulated by an intercellular quorum sensing mechanism involving the synthesis and detection of two signaling molecules, one of which is N-hydroxy butanoyl-L-homoserine lactone and the other of which is uncharacterized. Indirect evidence has suggested that virulence, associated with a toxic extracellular protein, and l...

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Characterization of Tri- and Tetranucleotide Microsatellites in the Black Tiger Prawn, Penaeus monodon

In order to characterize triand tetranucleotide microsatellites in the black tiger prawn, Penaeus monodon, a genomic library of P. monodon was screened with the repeats (GAA)n, (GATA)n, (GGAT)n, (GGAA)n and (CACC)n. In the 79 positive clones isolated, the repeats (GATA)n was found at the highest frequency, followed by the (GAA)n while the other sequence types were rare or not found. Sequencing ...

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ژورنال

عنوان ژورنال: Diseases of Aquatic Organisms

سال: 1994

ISSN: 0177-5103,1616-1580

DOI: 10.3354/dao020159